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KMID : 0385020000160010049
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Korean Journal of Laboratory Animal Science
2000 Volume.16 No. 1 p.49 ~ p.57
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The Growth and Functional Changes of Macrophages by Ciprofloxacin and Oxolinic Acid
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이현숙/Lee, Hyun Sook
박관하/송진숙/김희연/양승돈/심수연/이상달/Park, Kwan Ha/Song, Jin Sook/Kim, Hee Youn/Yang, Sung Don/Sim, Su Yeun/Rhee, Sang Dal
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Abstract
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The effects of ciprofloxacin(CIP) and oxolinic acid(OXO) on macrophage growth and functions were investigated using bone marrow-derived macrophages (BMDM). Bone marrow cells were cultured to become mature BMDM using L929ce11-conditioned medium(LCCM) as a source of macrophage growth factor (CSF-1) in the presence of 0, 10, 100, 500, and 1000/㎖ of drugs. At high concentrations of drugs (500 and 1000 ㎍/㎖), both CIP and OXO suppressed cell growth, whereas lower concentrations did not significantly affect cell growth. At day 4, which normally shows the highest proliferation of cells, high concentrations of drugs suppressed cell proliferation most profoundly. The number of adherent cells was significantly reduced and differentiation was apparently blocked by these drugs. The effects of these drugs to nitric oxide(NO) production by macrophages were monitored on 1, 2, and 3 days after stimulation by IFN-γ (100U/㎖) and LPS (10 ng/㎖). When the drugs were added after completion of BMDM culture, highest concentrations of OXO stimulated BMDM to produce more NO than control. These antibacterials suppressed NO production when they were added from the beginning of bone marrow cell culture except 500 ㎍/㎖ of OXO, which showed almost the same NO production as control. These results suggest that BMDM is a good model system for alternative toxicological research and NO production is a sensitive parameter of this system.
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KEYWORD
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